The Biomek i3 automated workstation optimizes cell transfection with precise liquid handling, tiling for efficient plate processing, and HEPA filtration for a clean, controlled environment. Its open deck accommodates plates, reservoirs, and accessories for media exchange and reagent addition. With intuitive software and customizable methods, the Biomek i3 reduces manual steps, minimizes variability, and supports high-throughput or research-scale experiments—delivering reliable transfection efficiency with contamination control.